دراسة الانترلوكين 12 والغلولوبين المناعي نوع E وعلاقتهما في شدة الربو القصبي عند الأطفال

Study of serum interleukin-12 and total  serum immunoglobulin-E in relation to bronchial asthma severity in childhood

Sattar jabbar Rahi- M.sc. Microbiology (immunology )

Iraq / Kerbala /Kerbala university / Collage of Medicine /  Department of Microbiology .

Sattar_j1972@yahoo.com

Abstract

Bronchial asthma is a major public health concern affecting 100-150 million people worldwide. Elevated total serum immunoglobulin E (IgE) is considered as an objective marker of allergy and has been associated with a number of respiratory disorder.Interleukin-12(IL-12) is a key cytokine involved in regulating the balance between TH1and TH2 cells by promoting TH1 response .Reduced capacity to produce this cytokine could lead aberrant TH2 development .The objective  in this to find if any correlation between serum IgE level and serum interleukin-12 with severity of bronchial asthma for both the definitive diagnosis and the  therapeutic strategy. A total of fifty patients with age 12years and below( 28 males and 22 females) and twenty five of case controls with matched age and sex were randomly selected from apparently healthy individuals from January to June of  2010 who were attending the outpatient department in Kerbala teaching  pediatrics hospital with symptoms suggestive of bronchial asthma .The patients who were not on any anti asthma medication in previous one week were included in the study. Blood sample was taken from each patient at time of attending , sera were separated and kept frozen at -20⁰C until used .Serum interleukin -12 and total  IgE levels were done by solid phase enzyme –linked immunosorbent assay (ELISA). A significant decrease of serum levels of IL-12 was found in bronchial asthmatic children compared with normal controls .This significant decrease of IL-12was observed in sever asthmatic patients compared with mild and moderate cases. However there is increase of mean serum total  IgE in bronchial asthmatic children compared to healthy control groups .mean serum IgE levels were increased  as the severity of asthma increased . The present study suggested that the increased  serum  total levels IgE in bronchial asthmatic patients beside decreasing the serum interleukin-12 could be considered as a key component in bronchial asthma pathogenesis and hence their therapeutic manipulation may be help in bronchial asthma management